Posters
Suitable reference genes for Real-Time PCR in gene expression studies with colorectal, lung and ovary clinical sample.
Classical housekeeping genes, such as 18S, GAPDH, HPRT1, GUSB, PGK1 are routinely used as endogenous references genes1-7 to determine differentially expressed genes in cancer studies. However, recent reports show that they could present serious differences between tissues, cell lines or clinical samples, which may lead to unreliable results and consequently misinterpretations. This study was focused on the selection of suitable reference genes for real time PCR in the clinical samples studied.
